Little-known microscopic and normal forest mushrooms are united that they require ready organic power supply. Suction comes from substrate of nutrients through mycelium. On mycelium under favorable conditions fruit bodies are formed. There is special process of receiving mycelium.
It is required to you
- - beer mash;
- - agar-agar;
- - test tubes;
- - cotton and gauze traffic jams;
- - torch;
- - steel wire;
- - traffic jams;
- - hydrogen peroxide;
- - shampinyonny compost;
- - bank with metal cover;
- - autoclave.
Instruction
1. As nutrient medium it is recommended to use mash agar. That take to prepare it liter of beer mash (8 ° across Ballingu) and add 20 grams of agar-agar. You cook before full dissolution. Hot pour mash agar on test tubes, filling them on 1/3 volumes. Close cotton traffic jams and sterilize 30 minutes with pressure of 1.5 atmospheres and temperature of 101 °C. Upon completion of sterilization install test tubes on table, it is strong them having inclined. Wednesday should not reach stopper of 4 cm, and, stiffening, to have big surface.
2. When Wednesday stiffens, in sterile conditions bring part of fruit mushroom or disputes in test tube. It is possible to make it by means of steel wire or knitting needle which end needs to be bent on 10 cm and to grind off. Before application it needs to be calcinated over fire. Pull down mushroom in half and cut out from the top share of leg needle mushroom fabric piece. Dip it into hydrogen peroxide. Then, holding test tube with Wednesday over torch flame, open it and place mushroom fabric piece on Wednesday.
3. Burn stopper over fire and zakuporta it test tube. Do not put stopper on table at all, and hold with little finger. For the period of overgrowing put test tube in the thermostat or in the dark place where the air temperature of 24 °C. In two weeks the mycelium will master nutrient medium, and it can be made multiple copies further.
4. To prepare compost mycelium which is used at cultivation of champignons take shampinyonny compost (already ready) and on the technology described above, place it in 3-liter jar. Fill banks on 2/3, condense substrate. In the center of compost make opening of Ø of 3 cm.
5. Make the same opening in metal cover and roll up it to bank. Insert cotton and gauze stopper into opening. Sterilize the jar in the autoclave with pressure of 2 atmospheres within 2 hours. Place substrate to cool down up to 24 °C to the sterile room. When cools down, bring the pure culture of mushroom which is grown up on nutrient medium in bank.